Acetylation assays were carried out as beforehand explained [five]. HEK293T, transfected with the indicated plasmids, had been lysed and overexpressed Gli2 was immunoprecipitaded with anti Myc (9E10) AC beads (Santa Cruz, sc-40 AC) NIH3T3 cells ended up lysed and endogenous Gli2 was immunoprecipitated with goat anti-Gli2 (one:one hundred, R&D programs)
Immediately after immunoblott acetylation was detected with rabbit polyclonal anti acetyl lysine (1:a thousand,...