Y J, Thorup T, et al. Molecular mapping of capsaicinoid biosynthesis genes and quantitative trait loci evaluation for capsaicinoid content material in Capsicum. Theoretical and Applied Genetics 108: 79 86. 19. Kim M, Kim S, Kim S, Kim B-D Isolation of cDNA clones differentially accumulated in the placenta of pungent buy Human parathyroid hormone-(1-34) pepper by suppression subtractive hybridization. Molecules and Cells 11: 213219. 20. Stewart C, Kang B-C, Liu K, Mazourek M, Moore SL, et al. The Pun1 gene for pungency in pepper encodes a putative acyltransferase. The Plant Journal 42: 675688. 21. Stewart C, Mazourek M, Stellari GM, O’Connell MA, Jahn M Genetic handle of pungency in C. chinense through the Pun1 locus. Journal of Experimental Botany 58: 979991. 22. Stellari GM, Mazourek M, Jahn MM Contrasting modes for loss of pungency involving cultivated and wild species of Capsicum. Heredity 104: 460 471. 23. Hill TA, Ashrafi H, Reyes-Chin-Wo S, Yao J, Stoffel K, et al. Characterization of Capsicum annuum Genetic Diversity and Population Structure Determined by Parallel Polymorphism Discovery using a 30K Unigene Pepper GeneChip. PLoS A single 8: e56200. 24. Han K, Jeong H-J, Sung J, Keum Y, Cho M-C, et al. Biosynthesis of capsinoid is controlled by the Pun1 locus in pepper. Molecular Breeding 31: 537548. 25. Yumnam J, Tyagi W, Pandey A, Meetei NT, Rai M Evaluation of Genetic Diversity of Chilli Landraces from North Eastern India Depending on 26. 27. Morphology, SSR Markers and also the Pun1 Locus. Plant Molecular Biology Reporter 30: 14701479. Bennett DJ, Kirby GW Constitution and biosynthesis of capsaicin. Journal from the Chemical Society C: Organic: 442446. Collins MD, Wasmund LM, Bosland PW Enhanced system for quantifying capsaicinoids in Capsicum using high-performance liquid chromatography. HortScience 30: 137139. Zewdie Y, Bosland PW Capsaicinoid profiles are usually not fantastic chemotaxonomic indicators for Capsicum species. Biochemical Systematics and Ecology 29: 161169. Iwai K, Suzuki T, Fujiwake H Formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues, in Capsicum annuun var. annuun cv. Karayatsubusa at different growth stages just after flowering. Agricultural and Biological Chemistry 43: 24932498. Rozen S, Skaletsky HJ Primer3. Out there: http://biotoolsumassmededu/ bioapps/primer3_wwwcgi. Wheelan SJ, Church DM, Ostell JM Spidey: a tool for mRNA-togenomic alignments. Genome Analysis 11: 19521957. Tamura K, Emixustat (hydrochloride) Peterson D, Peterson N, Stecher G, Nei M, et al. MEGA5: Molecular Evolutionary Genetics Evaluation using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Techniques. Molecular Biology and Evolution 28: 27312739. Librado P, Rozas J DnaSP v5: a computer software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 14511452. Higo K, Ugawa Y, Iwamoto M, Korenaga T Plant cis-acting regulatory DNA components database: 1999. Nucleic Acids Investigation 27: 297300. Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, et al. The 12926553 Structure of Haplotype Blocks in the Human Genome. Science 296: 22252229. Fallin D, Schork NJ Accuracy of Haplotype Frequency Estimation for Biallelic Loci, by means of the Expectation-Maximization Algorithm for Unphased Diploid Genotype Information. The American Journal of Human Genetics 67: 947 959. Abburi L Linkage disequilibrium and population structure evaluation among Capsicum annuum L. cultivars for use in association mapping. WV, USA: West Virginia State University. Holm S A simple sequentially rejective several test procedure. Scandinavian Journ.Y J, Thorup T, et al. Molecular mapping of capsaicinoid biosynthesis genes and quantitative trait loci analysis for capsaicinoid content in Capsicum. Theoretical and Applied Genetics 108: 79 86. 19. Kim M, Kim S, Kim S, Kim B-D Isolation of cDNA clones differentially accumulated in the placenta of pungent pepper by suppression subtractive hybridization. Molecules and Cells 11: 213219. 20. Stewart C, Kang B-C, Liu K, Mazourek M, Moore SL, et al. The Pun1 gene for pungency in pepper encodes a putative acyltransferase. The Plant Journal 42: 675688. 21. Stewart C, Mazourek M, Stellari GM, O’Connell MA, Jahn M Genetic manage of pungency in C. chinense via the Pun1 locus. Journal of Experimental Botany 58: 979991. 22. Stellari GM, Mazourek M, Jahn MM Contrasting modes for loss of pungency between cultivated and wild species of Capsicum. Heredity 104: 460 471. 23. Hill TA, Ashrafi H, Reyes-Chin-Wo S, Yao J, Stoffel K, et al. Characterization of Capsicum annuum Genetic Diversity and Population Structure According to Parallel Polymorphism Discovery having a 30K Unigene Pepper GeneChip. PLoS One 8: e56200. 24. Han K, Jeong H-J, Sung J, Keum Y, Cho M-C, et al. Biosynthesis of capsinoid is controlled by the Pun1 locus in pepper. Molecular Breeding 31: 537548. 25. Yumnam J, Tyagi W, Pandey A, Meetei NT, Rai M Evaluation of Genetic Diversity of Chilli Landraces from North Eastern India Determined by 26. 27. Morphology, SSR Markers along with the Pun1 Locus. Plant Molecular Biology Reporter 30: 14701479. Bennett DJ, Kirby GW Constitution and biosynthesis of capsaicin. Journal from the Chemical Society C: Organic: 442446. Collins MD, Wasmund LM, Bosland PW Enhanced process for quantifying capsaicinoids in Capsicum using high-performance liquid chromatography. HortScience 30: 137139. Zewdie Y, Bosland PW Capsaicinoid profiles are certainly not good chemotaxonomic indicators for Capsicum species. Biochemical Systematics and Ecology 29: 161169. Iwai K, Suzuki T, Fujiwake H Formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues, in Capsicum annuun var. annuun cv. Karayatsubusa at distinctive development stages right after flowering. Agricultural and Biological Chemistry 43: 24932498. Rozen S, Skaletsky HJ Primer3. Accessible: http://biotoolsumassmededu/ bioapps/primer3_wwwcgi. Wheelan SJ, Church DM, Ostell JM Spidey: a tool for mRNA-togenomic alignments. Genome Research 11: 19521957. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, et al. MEGA5: Molecular Evolutionary Genetics Analysis making use of Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Techniques. Molecular Biology and Evolution 28: 27312739. Librado P, Rozas J DnaSP v5: a application for comprehensive evaluation of DNA polymorphism information. Bioinformatics 25: 14511452. Higo K, Ugawa Y, Iwamoto M, Korenaga T Plant cis-acting regulatory DNA components database: 1999. Nucleic Acids Investigation 27: 297300. Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, et al. The 12926553 Structure of Haplotype Blocks inside the Human Genome. Science 296: 22252229. Fallin D, Schork NJ Accuracy of Haplotype Frequency Estimation for Biallelic Loci, by way of the Expectation-Maximization Algorithm for Unphased Diploid Genotype Information. The American Journal of Human Genetics 67: 947 959. Abburi L Linkage disequilibrium and population structure evaluation amongst Capsicum annuum L. cultivars for use in association mapping. WV, USA: West Virginia State University. Holm S A simple sequentially rejective various test process. Scandinavian Journ.