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VASP monoclonal antibody (IE273)
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VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization: the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a proline-rich motif, a more divergent proline-rich central domain (which is responsible for profilin binding), and a conserved C-terminal EVH2 domain. Particularly high VASP levels are present in platelets, although VASP is expressed in a wide variety of cell types and tissues.1622902-68-4 custom synthesis In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions.2374971-81-8 supplier From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp.PMID:27771104 surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
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| Alternative Name Vasodilator stimulated phosphoprotein | Application ICC, IP, WB | Clone IE273 | Formulation Liquid. In PBS containing 2mg/ml BSA and 0.02% sodium azide. | Host Mouse | Immunogen Purified human VASP (vasodilator stimulated phosphoprotein). | Isotype IgG1 | Recommendation Dilutions/Conditions Immunocytochemistry (1-5µg/ml)Western Blot (0.01-0.25µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Bovine, Human, Porcine, Rabbit | Specificity Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of VASP. | UniProt ID P50552 | Unit of Measure (UM) µg
