Product Name :
FLAG Peptide
CAS NO. :
98849-88-8
Formula :
C41H60N10O20
Molecular Weight::
1012.97
Target:
others
Serial Number:
Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
Short serial number :
DYKDDDDK
Purity:
95%
Description :
FLAG consists of 8 amino acids, and this tag can be removed by the addition of enterokinase treatment.FLAG peptide has an enterokinase cleavage site that can be used for antibody-mediated recognition and purification of recombinant proteins. FLAG peptide is a highly acidic octapeptide that can be fused to the N-terminus to the protein of interest. As a very hydrophilic peptide, Flag-tag has a high surface potential. In the presence of Ca2+, the marker fusion protein is captured by an immunoaffinity column and eluted with EDTA at low concentrations, neutral pH, and near-physiological conditions.FLAG peptide, an eight amino acids peptide with an enterokinase-cleavage site, is a frequently applied hydrophilic and immunogenic.
References:
[1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65. [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.
Related websites: https://www.medchemexpress.com/antibodies.html
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