Ession. Scale bars: 10 mAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptNature. Author manuscript; offered in PMC 2022 October 06.Konstantinides et al.PageAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptExtended Data figure 6: Added genetic interactions amongst tTFs(a) Diagram of genetic interactions among tTFs in medulla neuroblasts. Magenta dashed arrows inside the diagram indicate genetic interactions determined with misexpression of tTFs that confirm previous outcomes from MARCM and knock-down experiments. Orange dashed arrows indicate genetic interactions uncovered with misexpression experiments. Blue dashed arrows indicate genetic interactions determined with misexpression experiments that happen to be opposite to preceding MARCM or knock-down experiments. (b) Left: In opa mutant clones (GFP: green), Oaz just isn’t expressed, suggesting that Opa is essential for the activation of Oaz. Suitable: Accordingly, oaz neurons are lost in opa mutant clones. (c) Slp inhibits the expression of Oaz. In slp mutant clones (GFP: green), Oaz remains expressed in older neuroblasts. (d) In cells misexpressing Hth driven by Vsx-Gal4 (GFP: green), Erm expression is not impacted, indicating that Hth will not activate Erm. (e) In cells misexpressing Hth driven by Vsx-Gal4 (GFP: green), Opa expression is just not affected, indicating that Hth doesn’t activate Opa. (f) In cells misexpressing Ey driven by Vsx-Gal4 (GFP: green), Hbn expression is activated and Scro expression repressed.Nature. Author manuscript; offered in PMC 2022 October 06.Konstantinides et al.Web page(g) In cells misexpressing Hbn driven by Vsx-Gal4 (GFP: green), Slp expression will not be activated earlier. White arrowhead indicates low levels of Hbn protein being misexpressed early in comparison with adjacent wildtype tissue. (h) In cells misexpressing Scro driven by Vsx-Gal4 (GFP: green), D expression is not activated earlier. White arrowhead indicates low levels of Scro protein being misexpressed early in comparison to adjacent wildtype tissue. (i) In cells misexpressing D driven by Vsx-Gal4 (GFP: green), both Scro and BarH1 expression are repressed. (j) In cells misexpressing Tll driven by Vsx-Gal4 (GFP: green), both D and BarH1 expression are repressed. Scale bars: ten mAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptExtended Information figure 7: Temporal transcriptions elements are inherited in neurons and regulate neuronal diversity(a) UMAP plots showing the expression of all temporal transcription elements in the building Drosophila optic lobe. (a) Hth maintains its expression in a number of their neuronal progeny, for instance Mi1, Pm1, and Pm2. (a’) Alternatively, ey, D, erm and Scro not only retain their expression in some neuronal progeny, however they are also activated independently in neuronal varieties that come from various temporal windows.Biotin-azide Protocol Hence, mostNature.3-Methylcytidine custom synthesis Author manuscript; out there in PMC 2022 October 06.PMID:24576999 Konstantinides et al.Pageof them are expressed in both early- and late-born neuronal varieties (see also Supplementary Table 1). (a”) Slp1, opa, BarH1, Oaz, and tll usually do not preserve their transcription in neurons. On the other hand, applying their expression in GMCs in the root of neuronal branches, we can predict neuronal forms that come from these temporal windows (such Tm1, Tm2, and Tm4 for Opa). (a”’) Ultimately, hbn will not be maintained in neuronal progeny in the Hbn+ neuroblasts, however it is activated independently in later-born neurons (which include T1). Neuron.
