T/12/1/Table 3 Erythrocyte membrane fatty acid composition in LZR and OZR rats fed CON, FLAX, FISH, or SDA diets for 12 PRMT3 Inhibitor Gene ID weeksFatty acid ( of total) LA[18:two(n-6)]Lean CON 9.50 ?0.28 0.04 ?0.07a 0.04 ?0.02 0.03 ?0.05 25.63 ?0.51 0.05 ?0.11a 1.02 ?0.16 two.51 ?0.14 47.53 ?0.39 9.62 ?0.39 3.63 ?0.19 41.23 ?0.aObese FISH 9.72 ?0.28 0.ten ?0.07a 0.04 ?0.02 0.03 ?0.05 15.90 ?0.50 5.14 ?0.11f three.74 ?0.16 5.80 ?0.14 47.17 ?0.38 11.00 ?0.39 14.81 ?0.19 32.76 ?0.dMain effects FISH SDA six.73 ?0.31 0.61 ?0.08bc 0.20 ?0.02 0.40 ?0.05 18.13 ?0.56 3.98 ?0.12e 4.64 ?0.18 3.28 ?0.15 47.31 ?0.43 12.36 ?0.43 12.88 ?0.21 30.67 ?0.eFLAX 9.61 ?0.28 0.43 ?0.07b 0.04 ?0.02 0.03 ?0.05 22.21 ?0.51 1.06 ?0.11b three.67 ?0.16 3.74 ?0.14 47.58 ?0.39 9.94 ?0.39 eight.92 ?0.19 37.23 ?0.cdSDA 8.26 ?0.29 0.64 ?0.07c 0.30 ?0.02 0.40 ?0.05 20.67 ?0.52 two.80 ?0.11d five.44 ?0.17 two.31 ?0.14 47.49 ?0.40 10.14 ?0.40 11.63 ?0.20 33.01 ?0.eCON 7.76 ?0.30 0.14 ?0.07a 0.05 ?0.02 0.03 ?0.03 24.91 ?0.54 0.23 ?0.12a 1.19 ?0.17 2.91 ?0.15 47.35 ?0.41 11.91 ?0.42 4.48 ?0.20 38.61 ?0.aFLAX 7.97 ?0.29 0.70 ?0.07c 0.04 ?0.02 0.02 ?0.05 21.1 ?0.51 1.45 ?0.11c three.08 ?0.17 four.33 ?0.14 47.23 ?0.39 12.12 ?0.40 9.59 ?0.19 35.33 ?0.bGenotype P 0.0001 P = 0.24 P = 0.78 P = 0.74 P = 0.056 P = 0.0017 P = 0.052 P = 0.0010 P = 0.93 P = 0.0006 P = 0.Diet P 0.iInteraction P = 0.8.21 ?0.29 0.21 ?0.07a 0.04 ?0.02 0.03 ?0.05 14.80 ?0.52 5.41 ?0.12f three.17 ?0.18 six.50 ?0.14 47.67 ?0.40 12.12 ?0.40 15.31 ?0.20 31.33 ?0.bcALA[18:3(n-3)] GLA[18:three(n-3)]P 0.0001ii P 0.iiiP = 0.P = 0.52 P = 0.76 P = 0.SDA[18:4(n-3)] AA[20:four(n-6)]P 0.0001iii P 0.ivEPA[20:five(n-3)] DPA[22:5(n-3)]P 0.0001v P 0.0001 P = 0.99 P = 0.11 P 0.v viP 0.0001 P = 0.DHA[22:six(n-3)] SFA MUFA n3PUFA n6PUFAP 0.0001viiP = 0.ten P = 0.56 P = 0.23 P = 0.ten P = 0.P = 0.P 0.0001ivDifferent letters represent significance amongst indicates as determined by Bonferroni correction (P 0.05). Genotype Effect: Lean Obese; Obese Lean. Diet plan Impact: iCON, FLAX, FISH SDA; iiSDA, FLAX CON, FISH; iiiSDA CON, FLAX, FISH; ivCON FLAX SDA FISH; vFISH SDA FLAX CON; viSDA FLAX, FISH CON;viiFISH FLAX SDA, CON.Page 6 ofCasey et al. Lipids in Well being and Illness 2013, 12:147 lipidworld/content/12/1/Page 7 ofDHA, and AA content material was reduce with FLAX when compared with SDA (P 0.0001).Subcutaneous ATFigure 2 Erythrocyte membrane Omega-3 Index in LZR and OZR rats fed CON, FLAX, FISH, or SDA diets for 12 weeks. Omega-3 Index ( EPA + DHA) was calculated from RBC membranes as described [22]. All graphed values represent LS interaction means ?SE. Letters represent important differences among suggests as determined by the Bonferroni correction (n = 6, Genotype effect, P 0.0001 (Obese Lean); Diet regime impact, P 0.0001 (FISH SDA FLAX CON); DietGenotype interaction, P 0.0001).The N-type calcium channel Inhibitor custom synthesis percentage of ALA, SDA, MUFA, n3PUFA had been reduced; whereas, the percentage of DPA, DHA, and SFA had been greater in subcutaneous AT of obese vs. lean rodents (Table 7; Genotype, P 0.0001). The percentage of DPA and MUFA was higher; while the percentage of ALA and n3PUFA were reduced in subcutaneous AT with all n3PUFA-enriched diets (Diet plan, P 0.0001). Equivalent to epidydimal AT, the percentage of EPA, DPA, DHA, and SFA were greater with FISH vs. FLAX or SDA (Eating plan, P 0.0001). Nonetheless, the percentage of SDA, AA, and n3PUFA had been greater with SDA vs. FISH or FLAX (Eating plan, P 0.0001). When compared with FLAX, the percentage of EPA, DPA, and DHA were reduced with SDA (Diet, P 0.0001).Soleus muscleFISH vs. FLAX or SDA (Eating plan, P 0.05). Alterna.
