Served 15 amino sequence momotif that is lysine-rich (EKKGIMDKIKEKLPG), which can be named
Served 15 amino sequence momotif which is lysine-rich (EKKGIMDKIKEKLPG), which is called the K-segment; a Y-segtif that is lysine-rich (EKKGIMDKIKEKLPG), that is known as the K-segment; a Y-segment ment ML-SA1 custom synthesis positioned N terminus ([T/V]D[E/Q]YGNP); and an an S-(serine-track repeats) motif located in thein the N terminus ([T/V]D[E/Q]YGNP); andS-(serine-track repeats) motif [3]. [3]. K-segment is regarded the the segment of group II LEA proteins; it can be an an extenThe The K-segment is viewed as corecore segment of group II LEA proteins; it isextensive sive segment and lays or extra extra repeats, making amphiphilic -helixes in the Csegment and lays in onein a single orrepeats, building amphiphilic -helixes at the C-terminal terminal finish of the[26]. In relation torelation towards the arrangement and replication of those end on the proteins proteins [26]. Inside the arrangement and replication of those conserved conserved motif group II LEA proteins are classified classified into 5 subcategories: motif sequences, sequences, group II LEA proteins are into five subcategories: Kn, KnS, Kn, KnS, YnKn, YnSKn [30]. YnKn, SKn, and SKn, and YnSKn [30]. The proteins that possess only a a K-segment in their structural sequence belongthe proteins that possess only K-segment in their structural sequence belong to towards the K-subgroup of group II LEA proteins, andSK-subgroup comprises those group II LEA K-subgroup of group II LEA proteins, as well as the the SK-subgroup comprises these group II LEA proteins contain an S-segment accompanied by a K-segment in their their sequences proteins that that contain an S-segment accompanied by a K-segment in sequences (Fig(Figure 1) [3]. A conserved segment of group group II LEA proteins was found in the ure 1) [3]. A new new conserved segment of II LEA proteins was found at the N-termiN-terminus (DRGLFDFLGKK), which was termed the F-segment [31]. It was lately nus (DRGLFDFLGKK), which was termed the F-segment [31]. It was lately determined determined in plants as an overlooked motif of group II LEA proteins that hasfunctional in plants as an overlooked motif of group II LEA proteins that has prospective potential functional properties of binding to membranes protein molecules molecules [31]. properties of binding to membranes and also other along with other protein [31].Figure 1. Schematic representation from the positions of repeated sequences that differentiate group II Figure 1. Schematic representation in the positions of repeated sequences that differentiate group II LEA protein subgroups. The blocks represent the arrangement the motifs inside the corresponding LEA protein subgroups. The blocks represent the arrangement ofof the motifs within the corresponding subgroups of group II LEA proteins. The numbers on the appropriate indicate the tandem repeats of subgroups of group II LEA proteins. The numbers around the correct indicate the tandem repeats of each and every motif in various subgroups. The size array of group II LEA proteins is indicated in the major because the number of amino acid (aa) residues.The K-segments of group II LEA proteins interact with membranes and other proteins to modulate the proteins’ phase properties and conformational transitions [32]. The K-Biomolecules 2021, 11,5 ofsegment occurs in 1 to eleven copies inside a chain of amino acids [33]. It was reported in a study that the wheat group II LEA protein, DHN-5, shielded the activities of -glucosidase and lactate dehydrogenase (LDH) in vitro because of the Decanoyl-L-carnitine In Vitro presence of a K-segment in its amino acid.
