Alues and .cel files are available at the National Center for
Alues and .cel files are available at the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) repository http://www.ncbi.nlm.nih.gov/geo/ under Series Accession Number GSE10957. In addition, all scaled fluorescent intensity values are available in Supplemental Table 1.Gene expression profiling in mouse xenograft models Animal care was in accordance with NIH and institutional guidelines. For the A549 xenograft model, 1.7 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation [32]. For the A549 0 xenograft model, 1.5 million cells were used. Tumor and body weight measurements (6 or 7 mice per group) were performed three times per week once tumors became palpable. This occurred 41 versus 9 days post-implantation in 0 and parental AWestern blotting was performed using antibodies against GLUT1 (aka SLC2A1, ABT-737 cancer rabbit polyclonal), PGK1 (goat polyclonal), MTCO2 (mouse monoclonal), PDK1 (rabbit polyclonal), and DDIT4 (rabbit polyclonal) obtained from Santa Cruz Biotechnology, Molecular Probes, Stressgen, and Proteintech, respectively. All membranes were blotted with an anti-Hsc70 (mouse monoclonal, Santa Cruz Biotechnology) antibody to control for loading and transfer. HRP-conjugated goat anti-rabbit and goat antimouse secondary antibodies were from Pierce. HRP-conjugated donkey anti-goat secondary antibody was from Santa Cruz Biotechnology. HRP substrate was PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28854080 from Pierce. Bands were imaged and quantified in the linear range andPage 3 of(page number not for citation purposes)http://www.biomedcentral.com/1471-2164/9/Table 1: Transcripts where 90 of the variance in gene expression is explained by mtDNA statusAffymetrix Probe IDGene SymbolEntrez GeneIDGene Description0 Proportion of VarianceaF testb0 Vitro/A549 Vitro FCc Pd 0.0009 0.0057 0.0082 0.0088 0.0046 0.0045 0.0051 0.0090 0.0048 0.0041 0.0079 0.0245 0.0048 0.0291 0.0289 0.0045 0.0447 0.0141 0.0055 0.0524 0.0273 0.0435 0.0054 0.0186 0.0073 0.0069 0.0179 0.0491 0.0007 0.0 Vivo/A549 Vivo FCc Pd 3.7 2.7 2.7 13.1 9.3 4.8 10.2 4.2 2.9 9.7 3.1 1.6 11.5 1.6 2.7 12.7 1.9 11.0 10.9 1.9 2.4 1.6 11.2 1.5 1.7 1.7 2.2 1.8 -46.9 -14.8 0.0005 0.0002 0.0009 0.0007 0.0005 0.0010 0.0001 0.0020 0.0003 0.0001 0.0005 0.0014 0.0000 0.0042 0.0044 0.0001 0.0021 0.0004 0.0000 0.0015 0.0007 0.0030 0.0007 0.0032 0.0014 0.0050 0.0048 0.0018 0.0001 0.Up-regulated in 0 cells214247_s_at 209822_s_at 210105_s_at 221305_s_at 202242_at 205119_s_at 208596_s_at 219093_at 209040_s_at 206094_x_at 219959_at 201042_at 207126_x_at 203113_s_at 204802_at 215125_s_at 213587_s_at 217739_s_at 204532_x_at 212160_at 212307_s_at 203262_s_at 217738_at 201462_at 219762_s_at 212909_at 215025_at 201474_s_at 211600_at 205674_x_atDKK3 VLDLR FYN UGT1A8 TSPAN7 FPR1 UGT1A10 PID1 PSMB8 UGT1A6 PTHLH TGM2 UGT1A4 EEF1D RRAD UGT1A10 ATP6V0E2 PBEF1 UGT1A4 XPOT OGT FAM50A PBEF1 SCRN1 RPL36 LYPD1 NTRK3 ITGA3 MT-ND5 FXYD27122 7436 2534 54576 7102 2357 54575 55022 5696 54578 55034 7052 54657 1936 6236 54575 155066 10135 54657 11260 8473 9130 10135 9805 25873 116372 4916 3675 4540dickkopf homolog 3 very low density lipoprotein receptor FYN oncogene UDP glucuronosyltransferase 1 family tetraspanin 7 formyl peptide receptor 1 UDP glucuronosyltransferase 1 family hypothetical protein FLJ20701 proteasome subunit UDP glucuronosyltransferase 1 family p.
