Ig. 5j), further suggesting that the generated exocrine cells arise from true pancreatic progenitors. Altogether, we show the development of a protocol for pancreatic differentiation, which favours the production of acinar progenitors over the generation of both pancreatic endocrine and hepatic lineages.In vitro Differentiation of Transgenic RBPL-ES Cells: Cooperation with Ptf1a for Maximal Production of Digestive EnzymesThe fact that the exocrine transcription factor Rbpjl and a specific set of secretory enzymes, including Trypsin 3 (Prss3), Carboxyl ester lipase (Cel) and Ela1, induced at later stages of embryonic development [28] were increased at lower levels with the T19 protocol, suggested an incomplete activation of the exocrine differentiation program (Fig. 4A). To assess the ability of Rbpjl in regulating digestive enzyme gene modules to achieve advanced differentiation, lentiviral gene transduction was used to generate ESC cell lines with stable get JSH-23 expression of Rbpjl (RBPL-ES). An ESC line expressing GFP was used as KN-93 (phosphate) control (GFP-ES) (Fig. 6A). Characterization of transgene expression in RBPL-ES by RT-PCR, Western blot (not shown), and immunofluorescence confirmed the correct expression of ectopic Rbpjl with a nuclear localization (Fig. 6A). ESC clones expressing the highest Rbpjl mRNA levels as compared to adult pancreas were selected by qRT-PCR, such as clone #50, and behaved similarly in differentiation studies (Fig. 6B, and data not shown). Because the major known role of Rbpjl is as a Ptf1a transcriptional partner, the effect of superimposed Ptf1a overexpression during ESC exocrine differentiation in vitro was analyzed in parallel. We used a lentivirus expressing the Ptf1a gene fused to the mutated estrogen receptor ligand-binding domain (LvPtf1a-ER), allowing an inducible nuclear Ptf1a expression after Tamox addition. LvPtf1a-ER or LvGFP, as control, were introduced at the exocrine progenitor stage (stage 3, Fig. 1), to mimic more closely the timing when Ptf1a levels start to increase during pancreatogenesis. Transgene expression was observed in approximately 50 of ESC and addition of 2 mM Tamox was sufficient to relocate Ptf1a into the nuclear compartment (Fig. 6C). After 5 days of differentiation, control GFP-ESC infected with LvPtf1a-ER and exposed to Tamox expressed higher levels of Cpa1 mRNAs as compared with the same DMSO-treated cells (Fig. 6D). Of note, these later cultures (non-treated cells infected with LvPtf1a-ER) exhibited higher Ptf1a activity as compared to LvGFP infected cells, consistent with detectable nuclear amounts of Ptf1a (Fig. 6C). This likely reflects some leakage in Ptf1a-ER nuclear translocation. Therefore, we studied the conditions in which transgenic ES 23977191 cells were infected with LvGFP+Tamox versus LvPtf1a-ER+Tamox. Following the whole differentiation protocol and infection with LvGFP (Fig. 1), RBPL-ES cultures showed a limited but significant increase in a subset of secretory enzymes (Cpa1, Amyl, ChymoB1) in comparison with LvGFP-infected GFP-ES cultures (Fig. 7A), while the levels of endodermal and pancreatic progenitor markers at previous stages remained similar in both conditions (not shown). This differential increase in digestive enzymes between both cell lines was much stronger when cells were infected with LvPt1a-ER and treated with Tamox, while exhibiting similar levels of ectopic Ptf1a mRNAs (Fig. 6E). Notably, the combined action of Ptf1a andPancreatic Acinar Differentiation of Mouse E.Ig. 5j), further suggesting that the generated exocrine cells arise from true pancreatic progenitors. Altogether, we show the development of a protocol for pancreatic differentiation, which favours the production of acinar progenitors over the generation of both pancreatic endocrine and hepatic lineages.In vitro Differentiation of Transgenic RBPL-ES Cells: Cooperation with Ptf1a for Maximal Production of Digestive EnzymesThe fact that the exocrine transcription factor Rbpjl and a specific set of secretory enzymes, including Trypsin 3 (Prss3), Carboxyl ester lipase (Cel) and Ela1, induced at later stages of embryonic development [28] were increased at lower levels with the T19 protocol, suggested an incomplete activation of the exocrine differentiation program (Fig. 4A). To assess the ability of Rbpjl in regulating digestive enzyme gene modules to achieve advanced differentiation, lentiviral gene transduction was used to generate ESC cell lines with stable expression of Rbpjl (RBPL-ES). An ESC line expressing GFP was used as control (GFP-ES) (Fig. 6A). Characterization of transgene expression in RBPL-ES by RT-PCR, Western blot (not shown), and immunofluorescence confirmed the correct expression of ectopic Rbpjl with a nuclear localization (Fig. 6A). ESC clones expressing the highest Rbpjl mRNA levels as compared to adult pancreas were selected by qRT-PCR, such as clone #50, and behaved similarly in differentiation studies (Fig. 6B, and data not shown). Because the major known role of Rbpjl is as a Ptf1a transcriptional partner, the effect of superimposed Ptf1a overexpression during ESC exocrine differentiation in vitro was analyzed in parallel. We used a lentivirus expressing the Ptf1a gene fused to the mutated estrogen receptor ligand-binding domain (LvPtf1a-ER), allowing an inducible nuclear Ptf1a expression after Tamox addition. LvPtf1a-ER or LvGFP, as control, were introduced at the exocrine progenitor stage (stage 3, Fig. 1), to mimic more closely the timing when Ptf1a levels start to increase during pancreatogenesis. Transgene expression was observed in approximately 50 of ESC and addition of 2 mM Tamox was sufficient to relocate Ptf1a into the nuclear compartment (Fig. 6C). After 5 days of differentiation, control GFP-ESC infected with LvPtf1a-ER and exposed to Tamox expressed higher levels of Cpa1 mRNAs as compared with the same DMSO-treated cells (Fig. 6D). Of note, these later cultures (non-treated cells infected with LvPtf1a-ER) exhibited higher Ptf1a activity as compared to LvGFP infected cells, consistent with detectable nuclear amounts of Ptf1a (Fig. 6C). This likely reflects some leakage in Ptf1a-ER nuclear translocation. Therefore, we studied the conditions in which transgenic ES 23977191 cells were infected with LvGFP+Tamox versus LvPtf1a-ER+Tamox. Following the whole differentiation protocol and infection with LvGFP (Fig. 1), RBPL-ES cultures showed a limited but significant increase in a subset of secretory enzymes (Cpa1, Amyl, ChymoB1) in comparison with LvGFP-infected GFP-ES cultures (Fig. 7A), while the levels of endodermal and pancreatic progenitor markers at previous stages remained similar in both conditions (not shown). This differential increase in digestive enzymes between both cell lines was much stronger when cells were infected with LvPt1a-ER and treated with Tamox, while exhibiting similar levels of ectopic Ptf1a mRNAs (Fig. 6E). Notably, the combined action of Ptf1a andPancreatic Acinar Differentiation of Mouse E.
