Es of blend membranes were there by obtained and coded as K25C75, K50C50, and K75C25. Pure CS film and pure KGM film were prepared following the same protocol. The films were seal up for safekeeping after Co-60 1317923 irradiation. Film samples ofFigure 3. In vitro cumulative release profile of Gentamicin. From Poly (DEX-GMA/AAc) nanoparticles (A) and GNPs-CS/KGM (B). doi:10.1371/journal.pone.0066890.gAntibiotic Hemostatic First Aid Wound DressingFigure 4. The cross-section morphology of blending film with different KGM/CS ratio by SEM. doi:10.1371/journal.pone.0066890.gabout 100 mm thickness were coated with gold in 0.1Torr (0.13 mBar ) vacuum degrees. The cross section morphologies were observed on a Hitachi S-3400N SEM. The tensile strength (TS) and breaking elongation (E) of the films were measured on an Instron apparatus (Model 3342, Instron Corp., Canton, MA). The film (0.260.3 cm) were used in the stretch test under the stress of 1 and 2 N. Draft distance of clip was set as 20 mm with stretching speed of 50 mm?min21. TS and E were calculated following the formula below TS F=S; E (Lb {L0 )=L0 |100 F (N): maximum stress of stretching, S (mm2): initial sectional area of sample; L0: initial sample length; Lb: sample length at breakage (n = 6). The swelling behaviors of pure CS and CS/KGM blend films were studied according to the method described by Wang et al. [35]. The samples of a cylinder shape (diameter 30 mm, thickness 4 mm) were weighted (Wo) before immersing in PBS at 37uC 1315463 and immersed in PBS for different time intervals and weighted (Ws). The swelling degree in PBS (SDP) was calculated using thefollowing equation:Table 1. The water vapor transmission rate of different composition of CS/KGM films.WVTR (g?m22?day21) 3170 6 36 2580 6 90 2282 6 73 2950 6Composition (CS/KGM) (wt ) C25K75 C50K50 Title Loaded From File C75K25 CS Mean 6 S.D. (n = 6). doi:10.1371/journal.pone.0066890.tSDPWs -Wo |100(n 6) WoAntibiotic Hemostatic First Aid Wound DressingWhere Ws is the weight of the hydrogel swollen in PBS, Wo is the initial weight of hydrogel samples. The results are presented as a mean value with a standard deviation (n = 6). The water vapor transmission rate (WVTR) across the pure CS and CS/KGM blend films was determined to the method described by Tsao et al. [36]. The pure CS and CS/KGM blend films were cut into 20 mm620 mm with a thickness of 3 mm, and mounted on the mouth of cylindrical aluminium cups (14 mm diameter) containing 10 ml distilled water, and then placed in an incubator at 37uC. The WVTR was calculated using the following equation: WVTR Wo -Wt |106 (g=m2 =day) AIn vitro Gentamicin release from Poly (DEX-GMA/AAc) nanoparticlesDynamic dialysis method was employed to determine in vitro drug release profile of Gentamincin loaded Poly (DEX-GMA/ AAc) nanoparticles. The drug content in the drug-loaded nanoparticles was determined upon natural degradation of nanoparticles without enzyme and drug release caused by nanoparticles swelling due to AAc residue on the polymer backbone. An amount exactly weight (0.5 g) of drug-loaded nanoparticles dispersed in PBS (3 mL) was introduced into dialysis bag and dialysis against PBS (50 mL). Dialysate was incubated at 37uC under magnetically stirring. At each time interval, Dialysate (1.5 mL) was taken out and carefully transferred to a test tube. After each measurement, 1.5 mL of fresh buffer was added. After each measurement, 3 mL of fresh buffer was added. 5 mL Title Loaded From File derivatization regent was added into each sample and.Es of blend membranes were there by obtained and coded as K25C75, K50C50, and K75C25. Pure CS film and pure KGM film were prepared following the same protocol. The films were seal up for safekeeping after Co-60 1317923 irradiation. Film samples ofFigure 3. In vitro cumulative release profile of Gentamicin. From Poly (DEX-GMA/AAc) nanoparticles (A) and GNPs-CS/KGM (B). doi:10.1371/journal.pone.0066890.gAntibiotic Hemostatic First Aid Wound DressingFigure 4. The cross-section morphology of blending film with different KGM/CS ratio by SEM. doi:10.1371/journal.pone.0066890.gabout 100 mm thickness were coated with gold in 0.1Torr (0.13 mBar ) vacuum degrees. The cross section morphologies were observed on a Hitachi S-3400N SEM. The tensile strength (TS) and breaking elongation (E) of the films were measured on an Instron apparatus (Model 3342, Instron Corp., Canton, MA). The film (0.260.3 cm) were used in the stretch test under the stress of 1 and 2 N. Draft distance of clip was set as 20 mm with stretching speed of 50 mm?min21. TS and E were calculated following the formula below TS F=S; E (Lb {L0 )=L0 |100 F (N): maximum stress of stretching, S (mm2): initial sectional area of sample; L0: initial sample length; Lb: sample length at breakage (n = 6). The swelling behaviors of pure CS and CS/KGM blend films were studied according to the method described by Wang et al. [35]. The samples of a cylinder shape (diameter 30 mm, thickness 4 mm) were weighted (Wo) before immersing in PBS at 37uC 1315463 and immersed in PBS for different time intervals and weighted (Ws). The swelling degree in PBS (SDP) was calculated using thefollowing equation:Table 1. The water vapor transmission rate of different composition of CS/KGM films.WVTR (g?m22?day21) 3170 6 36 2580 6 90 2282 6 73 2950 6Composition (CS/KGM) (wt ) C25K75 C50K50 C75K25 CS Mean 6 S.D. (n = 6). doi:10.1371/journal.pone.0066890.tSDPWs -Wo |100(n 6) WoAntibiotic Hemostatic First Aid Wound DressingWhere Ws is the weight of the hydrogel swollen in PBS, Wo is the initial weight of hydrogel samples. The results are presented as a mean value with a standard deviation (n = 6). The water vapor transmission rate (WVTR) across the pure CS and CS/KGM blend films was determined to the method described by Tsao et al. [36]. The pure CS and CS/KGM blend films were cut into 20 mm620 mm with a thickness of 3 mm, and mounted on the mouth of cylindrical aluminium cups (14 mm diameter) containing 10 ml distilled water, and then placed in an incubator at 37uC. The WVTR was calculated using the following equation: WVTR Wo -Wt |106 (g=m2 =day) AIn vitro Gentamicin release from Poly (DEX-GMA/AAc) nanoparticlesDynamic dialysis method was employed to determine in vitro drug release profile of Gentamincin loaded Poly (DEX-GMA/ AAc) nanoparticles. The drug content in the drug-loaded nanoparticles was determined upon natural degradation of nanoparticles without enzyme and drug release caused by nanoparticles swelling due to AAc residue on the polymer backbone. An amount exactly weight (0.5 g) of drug-loaded nanoparticles dispersed in PBS (3 mL) was introduced into dialysis bag and dialysis against PBS (50 mL). Dialysate was incubated at 37uC under magnetically stirring. At each time interval, Dialysate (1.5 mL) was taken out and carefully transferred to a test tube. After each measurement, 1.5 mL of fresh buffer was added. After each measurement, 3 mL of fresh buffer was added. 5 mL derivatization regent was added into each sample and.
